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The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained.
Our Abpromise guarantee covers the use of ab8087 in the following tested applications.
|WB||Use at an assay dependent concentration. PubMed: 21645391|
ab8087 staining Endothelium in Human lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and methanol, and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50) overnight at 4°C. A TRITC-conjugated Goat anti-mouse IgG (1/50) was used as the secondary antibody.