Anti-Endothelin B Receptor 抗体 (ab117529)


  • 製品名
    Anti-Endothelin B Receptor antibody
    Endothelin B Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Endothelin B Receptor
  • アプリケーション
    適用あり: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide:


    conjugated to KLH, corresponding to C terminal amino acids 424-442 of Mouse Endothelin B Receptor.

  • ポジティブ・コントロール
    • Mouse lung tissue; Mouse brain lysate; Endothelin B Receptor transfected cells


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: 99% Whole serum
  • 精製度
    Whole antiserum
  • ポリ/モノ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab117529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/5000.
IHC-Fr 1/500 - 1/2000.
WB 1/5000. Predicted molecular weight: 50 kDa.
IHC-P 1/2000.
ICC/IF 1/5000.


  • 機能
    Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 組織特異性
    Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
  • 関連疾患
    Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
    Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
  • 翻訳後修飾
    Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABCDS antibody
    • Ednra antibody
    • EDNRB antibody
    • EDNRB_HUMAN antibody
    • Endothelin B receptor antibody
    • Endothelin B receptor precursor antibody
    • Endothelin receptor Non selective type antibody
    • Endothelin receptor non-selective type antibody
    • Endothelin receptor type B antibody
    • ET B antibody
    • ET-B antibody
    • ET-BR antibody
    • ETB antibody
    • ETBR antibody
    • ETRB antibody
    • Hirschsprung disease 2 antibody
    • HSCR antibody
    • HSCR2 antibody
    • OTTHUMP00000018534 antibody
    • OTTHUMP00000178736 antibody
    • WS4A antibody
    see all

Anti-Endothelin B Receptor antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor with ab117529.

  • Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor with ab117529 at 1/800 dilution. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (left panel) (1/800 dilution), and Anti-Endothelin B Receptor antibody (ab117529) (right panel) overnight at 4ºC. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1/800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.

    See Abreview

  • ab117529, at 1/5000 dilution, staining Endothelin B Receptor in HEK293 cells by Immunocytochemistry.
  • Anti-Endothelin B Receptor antibody (ab117529) at 1/5000 dilution + Mouse brain lysate

    Predicted band size : 50 kDa

Anti-Endothelin B Receptor antibody (ab117529) 使用論文

This product has been referenced in:
  • Gulati S  et al. Attenuation of opioid tolerance by ETB receptor agonist, IRL-1620, is independent of an accompanied decrease in nerve growth factor in mice. Heliyon 3:e00317 (2017). WB ; Mouse . Read more (PubMed: 28626808) »

See 1 Publication for this product

Product Wall

Immunocytochemistry/ Immunofluorescence
Human Cell (Blood)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 37°C
life cell staining

Abcam user community

Verified customer

投稿 Oct 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Mouse Tissue sections (lung)

Abcam user community

Verified customer

投稿 Jun 05 2013

I am sorry for the delay in getting back to you.
I now have confirmation that the antigen retrieval method we have used with the anti-Endothelin B Receptor antibody (ab117529) to obtain the image presented on the datasheet was citric acid buffer (...

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Thank you for contacting us and your interest in our products.
Our Endothelin Receptor B Polyclonal Antibody - product ab117529 is provided as undiluted antisera. This contains many other proteins other than the specific antibodies and quantificat...

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Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab84182 Anti-Endothelin B Receptor antibody - C-terminal in IHC-Fr zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörper...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde ab117529 Anti-Endothelin B Receptor antibody bisher noch nicht inIHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ic...

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