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KANDHGYDNFRSSNKYSSSconjugated to KLH, corresponding to C terminal amino acids 424-442 of Mouse Endothelin B Receptor.
Our Abpromise guarantee covers the use of ab117529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/500 - 1/2000.|
|WB||1/5000. Predicted molecular weight: 50 kDa.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor with ab117529.
Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor with ab117529 at 1/800 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.
ab117529 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"