製品の概要

  • 製品名Anti-Endothelin 1 antibody [TR.ET.48.5]
    Endothelin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TR.ET.48.5] to Endothelin 1
  • 特異性Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells. Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine).
  • アプリケーション適用あり: RIA, IP, Inhibition Assay, ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Dog, Human
  • 免疫原

    Full length protein corresponding to Human Endothelin 1 conjugated to Keyhole Limpet Haemocyanin (KLH).

  • エピトープStudies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.
  • ポジティブ・コントロール
    • human bowel

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
RIA 1/25000.
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
IHC-Fr 1/250.
IHC-P 1/250.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.

ターゲット情報

Anti-Endothelin 1 antibody [TR.ET.48.5] 画像

  • ab2786 labelling Endothelin 1 (green) in the secretion of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • ab2786 labelling Endothelin 1 (green) in the secretion of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • ab2786 labelling Endothelin 1 (green) in the secretion of HUVEC cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L)  was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 293T cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

  • Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

  • Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

  • Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) at 1/500 dilution + PC12 cell lysate at 25 µg

    Predicted band size : 24 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Figure 1 and Figure 2 show immunolocalization of ET-1 in human bowel using ab2786.

Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) 使用論文

This product has been referenced in:
  • Olgun NS  et al. BQ-123 Prevents LPS-Induced Preterm Birth in Mice via the Induction of Uterine and Placental IL-10. Toxicol Appl Pharmacol N/A:N/A (2014). Mouse . Read more (PubMed: 25230003) »
  • Xu XF  et al. Epigenetics of hyper-responsiveness to allergen challenge following intrauterine growth retardation rat. Respir Res 15:137 (2014). WB, IHC-P ; Rat . Read more (PubMed: 25391516) »

See all 13 Publications for this product

Product Wall

Thank you for your reply and further enqjuiry. I am sorry to confirm that the IHC image provided on the datasheet is the only one available at present. There are also 6 publications cited on the datasheet which have used this antibody. Ican suggest to ...

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Thank you for your message and for kindly providing the requested details. I am pleased to provide the following information: Endothelin -1 antibody ab2786 According to the image on the datasheet, this antibody has been successfully used in  hu...

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Thank you for your enquiry. With necrotic tissue, it is very likely that the proteins are already being broken down and degraded by the enzymes that are already present. I am sorry it would be very difficult to say and guarantee how much Endothelin...

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Thank you for your enquiry. I'm afraid we have no data regarding the detection of PreproET or Big ET using this antibody at this time. Both ET-1 and Big ET are contained within PreproET. Therefore I would expect that this antibody would detect PreproET...

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Thank you for your enquiry. It is not possible to determine the antibody concentration for this product, since it is ascites, and ascites contains proteins besides the antibody of interest. Ascites fluid is clarified by centrifugation to remove ...

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I am very sorry. I misunderstood your question. Yes, using mouse IgG1 will allow you to determine what antibodies found in mouse IgG1 will bind to your slides. If you see some binding then this is background that should not be confused with your ET-...

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Thank you for your enquiry. Ab2786 detects ET-1 in human tissues. This antibody shows little cross-reactivity to related proteins. The antigen is purified human endothelin-1. Studies suggest that this antibody binds to an epitope in the region...

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Thank you for your email. Since ab2786 has not yet been tested in Western blotting (to our knowledge), it's tricky to say what could be going on. At this point I would suggest varying the concentrations of both your primary and secondary antibodies, ma...

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