Anti-Endothelial Cell 抗体 [1F10] (Biotin) (ab51675)

製品の概要

  • 製品名
    Anti-Endothelial Cell antibody [1F10] (Biotin)
    Endothelial Cell 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [1F10] to Endothelial Cell (Biotin)
  • 標識
    Biotin
  • 特異性
    ab51675 recognizes an antigen expressed on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not stain any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent. On isolated cells, the antigen is found in variable numbers on freshly isolated umbilical vein endothelial cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40-70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercially available endothelial cell media apparently have no influence on the antigen expression.
  • アプリケーション
    適用あり: IHC-Frmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Cultured HUVECs.

  • ポジティブ・コントロール
    • Human placenta.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    1F10
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 1 µg/ml.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 関連性
      The endothelium is a single layer of cells lining various organs and cavities of the body, especially the blood vessels, heart, and lymphatic vessels.Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.

    プロトコール

    参考文献

    This product has been referenced in:
    • Goerdt S  et al. Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro. Exp Cell Biol 57:185-92 (1989). Read more (PubMed: 2599260) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for contacting us regarding your search for antibodies to endothelial phenotype.

    As we had spoken about, ab51675 is an excellent product against endothelial cell and is guaranteed to work in human samples in IHC-Fr. However as endo...

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    Thank you for contacting us.

    Yes, ab51675 is biotin conjugated. Biotin is not fluorescent so it does not have an excitation and anemission wavelength. If you would like to detect the target by fluorescence, you could use ab51675 withafluoresc...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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