Anti-Endothelial Cell 抗体 [1F10] (Biotin) (ab51675)


  • 製品名Anti-Endothelial Cell antibody [1F10] (Biotin)
    Endothelial Cell 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [1F10] to Endothelial Cell (Biotin)
  • 標識Biotin
  • 特異性ab51675 recognizes an antigen expressed on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not stain any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent. On isolated cells, the antigen is found in variable numbers on freshly isolated umbilical vein endothelial cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40-70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercially available endothelial cell media apparently have no influence on the antigen expression.
  • アプリケーション適用あり: IHC-Frmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Cultured HUVECs.

  • ポジティブ・コントロール
    • Human placenta.



  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名1F10
  • アイソタイプIgG2a
  • 研究分野


Our Abpromise guarantee covers the use of ab51675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 1 µg/ml.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 関連性The endothelium is the layer of thin specialized epithelium, comprising a simple squamous layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall (simple squamous epithelium). Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.

    Anti-Endothelial Cell antibody [1F10] (Biotin) (ab51675) 使用論文

    This product has been referenced in:
    • Goerdt S  et al. Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro. Exp Cell Biol 57:185-92 (1989). Read more (PubMed: 2599260) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us regarding your search for antibodies to endothelial phenotype.

    As we had spoken about, ab51675 is an excellent product against endothelial cell and is guaranteed to work in human samples in IHC-Fr. However as endo...

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    Thank you for contacting us.

    Yes, ab51675 is biotin conjugated. Biotin is not fluorescent so it does not have an excitation and anemission wavelength. If you would like to detect the target by fluorescence, you could use ab51675 withafluoresc...

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