製品の概要

  • 製品名
  • 製品の概要

    In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.

  • 特記事項

    In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.

  • アプリケーション
    適用あり: IHC-P, ICC/IF, IHC-Frmore details

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • バッファー
    Preservative: 0.08% Sodium azide
    Constituent: Avidin
  • 内容 15 ml
    Avidin Block 1 x 15ml
    Biotin Block 1 x 15ml
  • 研究分野
  • 関連性
    Some cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.

アプリケーション

Our Abpromise guarantee covers the use of ab64212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

画像

  • Immunohistochemical analysis staining HTRA1 in mouse bone tissue sections. Tissue sections were dewaxed, rehydrated and treated with Avidin/Biotin blocking kit (ab64212), 3% H2Oand normal swine serum. Tissue sections were then incubated with a polyclonal anti-HTRA1 antibody for 1 hour at 37°C. After PBS wash, tissue sections are incubated with biotinylated swine anti-rabbit IgG for 45minutes at 37°C and further incubated for 30 minutes after washing. With Vectastatin. Sections were developed using DAB and counterstained using Harris modified hematoxylin. 

参考文献

This product has been referenced in:
  • Filliat G  et al. Role of HTRA1 in bone formation and regeneration: In vitro and in vivo evaluation. PLoS One 12:e0181600 (2017). IHC . Read more (PubMed: 28732055) »
  • Planque SA  et al. Physiological IgM class catalytic antibodies selective for transthyretin amyloid. J Biol Chem 289:13243-58 (2014). Read more (PubMed: 24648510) »

See all 2 Publications for this product

レビューと Q&A


I can confirm that the Avidin Block and Biotin block provided with this kit come ready to use. They should not be diluted.

Once the sections are cut into sections and placed on a slide, the avidin and biotin blocking is done as part of th...

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Thank you for contacting us.

I would not recommend using the normal serum after biotin blocking for fear of introducing biotin to the samples. However if you would like, you may block with normal serum (5-10%) prior to biotin/avidin blocki...

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Thank you for your reply.

In this case I would recommend that you quench endogenous biotin/avidin in your tissue prior to staining. We recommend use of our Endogenous Avidin/Biotin Blocking Kit (ab64212).

After blocking with th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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