Anti-Endo G 抗体 [EP1665Y] (ab76122)


  • 製品名Anti-Endo G antibody [EP1665Y]
    Endo G 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1665Y] to Endo G
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues on the C terminus of human Endo G.

  • ポジティブ・コントロール
    • HepG2 cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.




Our Abpromise guarantee covers the use of ab76122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 関連性Endo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
    • 細胞内局在Mitochondrial
    • 参照データベース
    • 別名
      • EndoG antibody
      • Endonuclease G antibody
      • Endonuclease G mitochondrial antibody
      • EndonucleaseG antibody
      • FLJ27463 antibody
      • Mitochondrial endonuclease G antibody
      • NUCG_HUMAN antibody
      see all

    Anti-Endo G antibody [EP1665Y] 画像

    • Predicted band size : 36 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Endo G HAP1 cell lysate (20 µg)
      Lane 3: Mouse liver tissue lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

      ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/1000 dilution
      developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    Anti-Endo G antibody [EP1665Y] (ab76122) 使用論文

    ab76122 has not yet been referenced specifically in any publications.

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