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RabMAb

Anti-Endo G 抗体 [EP1665Y] (ab76122)

製品の概要

  • 製品名
    Anti-Endo G antibody [EP1665Y]
    Endo G 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1665Y] to Endo G
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Endo G (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HepG2 cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 関連性
      Endo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
    • 細胞内局在
      Cytoplasmic, Mitochondrial and Nuclear
    • 参照データベース
    • 別名
      • EndoG antibody
      • Endonuclease G antibody
      • Endonuclease G mitochondrial antibody
      • EndonucleaseG antibody
      • FLJ27463 antibody
      • Mitochondrial endonuclease G antibody
      • NUCG_HUMAN antibody
      see all

    画像



    • Predicted band size : 36 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Endo G HAP1 cell lysate (20 µg)
      Lane 3: Mouse liver tissue lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

      ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    This product has been referenced in:
    • Pardo R  et al. EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis. Endocrinology 157:3873-3887 (2016). Mouse . Read more (PubMed: 27547848) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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