製品の概要

  • 製品名Anti-Endo G antibody
    Endo G 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Endo G
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Endo G.

    (Peptide available as ab66560.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human Heart Tissue Mouse Spinal Cord Tissue Mouse Skeletal Muscle Tissue Rat Brain Tissue HeLa Whole Cell

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab66561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human Endo G peptide (ab9648).

ターゲット情報

  • 関連性Endo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
  • 細胞内局在Mitochondrial
  • 参照データベース
  • 別名
    • EndoG antibody
    • Endonuclease G antibody
    • Endonuclease G mitochondrial antibody
    • EndonucleaseG antibody
    • FLJ27463 antibody
    • Mitochondrial endonuclease G antibody
    • NUCG_HUMAN antibody
    see all

Anti-Endo G antibody 画像

  • All lanes : Anti-Endo G antibody (ab66561) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Rat Kidney Tissue Lysate
    Lane 4 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

Anti-Endo G antibody (ab66561) 使用論文

ab66561 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab66561.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"