Rabbit polyclonal to Encephalopsin
Synthesized peptide derived from an internal region of human Encephalopsin.
WB: Extracts from mouse brain cells.
ICC/IF: MCF-7 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody was affinity purified from rabbit antiserum by affinity chromatography
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 45 kDa.
1/500 - 1/1000.
May play a role in encephalic photoreception.
Strongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord.
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Information by UniProt
Western blot - Anti-Encephalopsin antibody (ab75285)
All lanes : Anti-Encephalopsin antibody (ab75285) at 1/500 dilution Lane 1 : Extracts from mouse brain cells Lane 2 : Extracts from mouse brain cells with immunising peptide at 10 µg Lysates/proteins at 10 µg per lane. Predicted band size: 45 kDa Observed band size: 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Encephalopsin antibody (ab75285)
ab75285 at 1:500-1:1000 staining Encephalopsin in MCF-7 cells by Immunofluorescence Image on right when blocking peptide added.
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