製品の概要

  • 製品名Anti-ENAH antibody
    ENAH 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ENAH
  • 特異性Detects endogenous levels of total ENAH protein.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthesized peptide derived from internal of human ENAH.

  • ポジティブ・コントロール
    • Extracts from HepG2 cells and human breast carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報ELISA: 1/10,000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 67 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
    • 組織特異性Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
    • 配列類似性Belongs to the Ena/VASP family.
      Contains 1 WH1 domain.
    • ドメインThe EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    • 翻訳後修飾NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ENA antibody
      • Enah antibody
      • ENAH_HUMAN antibody
      • MENA antibody
      • NDPP1 antibody
      • Protein enabled homolog antibody
      see all

    Anti-ENAH antibody 画像

    • All lanes : Anti-ENAH antibody (ab92849) at 1/500 dilution

      Lane 1 : Extracts from HepG2 cells
      Lane 2 : Extracts from HepG2 cells with peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 67 kDa
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab92849 at 1/50-1/100 dilution with (+) and without (-) immunising peptide.

    Anti-ENAH antibody (ab92849) 使用論文

    ab92849 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (neuron)
    Permeabilization Yes - 0.2%Triton
    Specification neuron
    Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Oct 13 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (COS7)
    Permeabilization Yes - 0.2%Triton
    Specification COS7
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Oct 07 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (brain)
    Loading amount 40 µg
    Specification brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    投稿 Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"