製品の概要

  • 製品名Anti-EMX2 antibody
    EMX2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EMX2
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Chicken, Cow, Dog, Human, Pig, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse EMX2.

    (Peptide available as ab109642.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Mouse brain and hippocampus tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
  • 組織特異性Cerebral cortex.
  • 関連疾患Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid.
  • 配列類似性Belongs to the EMX homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Empty spiracles homeobox 2 antibody
    • Empty spiracles homolog 2 (Drosophila) antibody
    • Empty spiracles homolog 2 antibody
    • Empty spiracles like protein 2 antibody
    • Empty spiracles-like protein 2 antibody
    • EMX 2 antibody
    • EMX2 antibody
    • EMX2_HUMAN antibody
    • Homeobox protein EMX 2 antibody
    • Homeobox protein EMX2 antibody
    see all

Anti-EMX2 antibody 画像

  • All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml

    Lane 1 : Mouse Hippocampus Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 300 kDa,74 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-EMX2 antibody (ab94713) 使用論文

ab94713 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (BRAIN)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 40 µg
Specification BRAIN
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Ms. kyungjoo seong

Verified customer

投稿 Nov 30 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (E14 mouse ES cells transduced with lentiviral EMX2)
Specification E14 mouse ES cells transduced with lentiviral EMX2
Permeabilization Yes - 10min Triton X-100 RT
Fixative Paraformaldehyde
Username

Mr. Albrecht

Verified customer

投稿 Dec 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"