Anti-EMX2 抗体 (ab94713)
Key features and details
- Rabbit polyclonal to EMX2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EMX2 antibody
EMX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EMX2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Dog, Pig, Zebrafish -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse brain and hippocampus tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94713の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
ターゲット情報
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機能
Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. -
組織特異性
Cerebral cortex. -
関連疾患
Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid. -
配列類似性
Belongs to the EMX homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 523601 Cow
- Entrez Gene: 2018 Human
- Entrez Gene: 13797 Mouse
- Entrez Gene: 499380 Rat
- Omim: 600035 Human
- SwissProt: Q17R00 Cow
- SwissProt: Q04743 Human
- SwissProt: Q04744 Mouse
see all -
別名
- Empty spiracles homeobox 2 antibody
- Empty spiracles homolog 2 (Drosophila) antibody
- Empty spiracles homolog 2 antibody
see all
画像
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All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml
Lane 1 : Mouse Hippocampus Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Additional bands at: 260 kDa (possible non-specific binding), 76 kDa (possible non-specific binding)
Exposure time: 16 minutes -
All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml
Lane 1 : Human Brain lysate
Lane 2 : Mouse Brain lysate
Lane 3 : NIH 3T3 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 minuteBlocking buffer: 2% BSA
Gel type: MES
Additional bands: 15 kDa (possible non-specific binding), 75 kDa (possible non-specific binding)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab94713 は 2 報の論文で使用されています。
- Rosebrock D et al. Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells. Nat Cell Biol 24:981-995 (2022). PubMed: 35697781
- Rysenkova KD et al. Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene (Plaur) and Its Molecular Target Mef2d. Front Mol Neurosci 15:865858 (2022). PubMed: 35875662