Anti-EMSY 抗体 [Y474] (ab32329)

製品の概要

  • 製品名Anti-EMSY antibody [Y474]
    EMSY 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y474] to EMSY
  • 特異性ab32329 recognises EMSY.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    corresponding to Human EMSY aa 1-100 (C terminal).
    Database link: Q7Z589
    (Peptide available as ab174721)

  • エピトープab32329 reacts with an epitope located in the C terminal region if EMSY.
  • ポジティブ・コントロール
    • MCF7 cells NIH/3T3 (human neuroblastoma) whole cell lysate C6 (rat glioma) whole cell lysate HeLa (human cervix adenocarcinoma) whole cell lysate
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 141 kDa.
IP 1/30.
  • 追加情報Is unsuitable for ICC/IF or IHC-P.
  • ターゲット情報

    Anti-EMSY antibody [Y474] 画像

    • All lanes : Anti-EMSY antibody [Y474] (ab32329) at 1/10000 dilution

      Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
      Lane 2 : C6 (rat glioma) whole cell lysate
      Lane 3 : Rat Ovary

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 141 kDa
      Observed band size : 141 kDa

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-EMSY antibody [Y474] (ab32329) at 1/10000 dilution

      Lane 1 : MCF-7 (human breast carcinoma) whole cell lysate
      Lane 2 : La (human cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 141 kDa
      Observed band size : 141 kDa

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

       

    • Anti-EMSY antibody [Y474] (ab32329) at 1/1000 dilution + MCF7 cell lysate

      Predicted band size : 141 kDa
      Observed band size : 141 kDa
    • Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    Anti-EMSY antibody [Y474] (ab32329) 使用論文

    ab32329 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. Rabbit monoclonal [Y474] to EMSY (ab32329) was raised using a synthetic peptide corresponding to residues near the C-terminal region of human EMSY isoform 1. The exact sequence is as follows: CGNVGQKKAEESPAEI I hope t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"