製品の概要

  • 製品名Anti-EMSY antibody
    EMSY 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to EMSY
  • 特異性This antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY.
  • アプリケーション適用あり: ICC/IF, WB, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to EMSY ENT domain:

    MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGDLTKEKKDLLG ELSKVLSISTER HRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRL

    , corresponding to amino acids 1-100 of Human EMSY - FPLC purified.

  • ポジティブ・コントロール
    • MCF-7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 141 kDa.
ICC Use at an assay dependent dilution.

ターゲット情報

Anti-EMSY antibody 画像

  • Lanes 1 - 2 : Anti-EMSY antibody (ab4579) at 1 µg/ml
    Lane 3 : Anti-EMSY antibody (ab123) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 141 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    Western blot of EMSY on MCF-7 cell lysate.

    Lane 1: ab4579 at 1ug/ml with 2% BSA blocking.

    Lane 2: ab4579 at 1ug/ml with 3% milk blocking.

    Lane 3: ab123 at 1ug/ml with 2% BSA blocking.

    Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.

     Lane 1 & 2 - Exposure time: 2 min.

    Lane 3 - Exposure time: 2 sec.

Anti-EMSY antibody (ab4579) 使用論文

ab4579 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"