Anti-EML4 抗体 (ab86062)
Key features and details
- Rabbit polyclonal to EML4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EML4 antibody
EML4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EML4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region between residues 150 and 200 of human EML4 (NP_061936.2)
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ポジティブ・コントロール
- HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab86062 was affinity purified using an epitope specific to EML4 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86062の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 109 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 109 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic. -
配列類似性
Belongs to the WD repeat EMAP family.
Contains 9 WD repeats. -
発生段階
Strongly overexpressed during mitosis. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 27436 Human
- Omim: 607442 Human
- SwissProt: Q96HE3 Human
- SwissProt: Q9HC35 Human
- Unigene: 593614 Human
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別名
- EML4 antibody
- C2orf2 antibody
- Echinoderm microtubule associated protein like 4 antibody
see all
画像
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All lanes : Anti-EML4 antibody (ab86062) at 1/0.04 dilution
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 109 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lin plastica stomach cancer tissue labelling EML4 with ab86062 at 1/200 (1µg/ml). Detection: DAB.
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Detection of EML4 by Western Blot of Immunprecipitate.
ab86062 at 0.4µg/ml staining EML4 in HeLa whole cell lysate immunoprecipitated using ab86062 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab86062 は 1 報の論文で使用されています。
- Nogueira E et al. A Non-Synonymous Point Mutation in a WD-40 Domain Repeat of EML5 Leads to Decreased Bovine Sperm Quality and Fertility. Front Cell Dev Biol 10:872740 (2022). PubMed: 35478957