Rabbit polyclonal to EML4
Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 150 and 200 of human EML4 (NP_061936.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab86062 was affinity purified using an epitope specific to EML4 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 109 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
Belongs to the WD repeat EMAP family.
Contains 9 WD repeats.
Strongly overexpressed during mitosis.
Cytoplasm > cytoskeleton.
Information by UniProt
Echinoderm microtubule associated protein like 4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (ab86062)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lin plastica stomach cancer tissue labelling EML4 with ab86062 at 1/200 (1µg/ml). Detection: DAB.
Western blot - EML4 antibody (ab86062)
All lanes :
Anti-EML4 antibody (ab86062) at 1/0.04 dilution
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Predicted band size:
Observed band size:
120 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - EML4 antibody (ab86062)
Detection of EML4 by Western Blot of Immunprecipitate.
ab86062 at 0.4µg/ml staining EML4 in HeLa whole cell lysate immunoprecipitated using ab86062 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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