製品の概要

  • 製品名
    Anti-EML3 antibody
  • 製品の詳細
    Rabbit polyclonal to EML3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region within the N terminal amino acids 180-229 (LLVRSGSTES RGGKDPLSSP GGPGSRRSNY NLEGISVKMF LRGRPITMYI) of human EML3 (NP_694997).

  • ポジティブ・コントロール
    • Human placenta lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    EML3 is a nuclear microtubule binding protein required for the correct alignment of chromosomes in metaphase. EML3 may modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • Echinoderm microtubule associated protein like 3 antibody
    • ELP 95 antibody
    • EMAP 3 antibody
    • EML 3 antibody
    • FLJ 35827 antibody
    • MGC 111422 antibody
    see all

画像

  • Anti-EML3 antibody (ab83821) at 1 µg/ml + human placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 95 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab83821 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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