製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to EME1
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1 - 583 of Human EME1 (AAH16470.1)

  • ポジティブ・コントロール
    • HeLa or Jurkat cell lysate. HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 配列類似性
    Belongs to the EME1/MMS4 family.
  • 細胞内局在
    Nucleus > nucleolus. Recruited to regions of DNA damage in S-phase cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6820428D13 antibody
    • Crossover junction endonuclease EME1 antibody
    • EC 3.1.22.- antibody
    • EME1 antibody
    • Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe) antibody
    • EME1, S. pombe, homolog of, 1 antibody
    • EME1_HUMAN antibody
    • essential meiotic endonuclease 1 homolog 1 (S pombe) antibody
    • Essential Meiotic Endonuclease 1 Homolog 1 antibody
    • essential meiotic endonuclease 1 homolog 2 antibody
    • Essential meiotic endonuclease 1, S. pombe, homolog of, 1 antibody
    • FLJ31364 antibody
    • hMMS4 antibody
    • homolog of yeast EME1 endonuclease antibody
    • MGC106543 antibody
    • MMS4 antibody
    • MMS4 homolog antibody
    • MMS4L antibody
    see all

画像

  • Anti-EME1 antibody (ab88878) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 63 kDa
  • Anti-EME1 antibody (ab88878) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 63 kDa
  • ab88878 at 10 µg/ml staining EME1 in HeLa cells by immunofluorescence.

参考文献

ab88878 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
1000 µg
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投稿 Jul 11 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

投稿 Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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