製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EME1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (ALKKSSPSLD SGDSDSEELP TFAFLKKEPS STKRRQPERE EKIVVVDISD) of human EME1 (NP_689676).

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 配列類似性
    Belongs to the EME1/MMS4 family.
  • 細胞内局在
    Nucleus > nucleolus. Recruited to regions of DNA damage in S-phase cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6820428D13 antibody
    • Crossover junction endonuclease EME1 antibody
    • EC 3.1.22.- antibody
    • EME1 antibody
    • Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe) antibody
    • EME1, S. pombe, homolog of, 1 antibody
    • EME1_HUMAN antibody
    • essential meiotic endonuclease 1 homolog 1 (S pombe) antibody
    • Essential Meiotic Endonuclease 1 Homolog 1 antibody
    • essential meiotic endonuclease 1 homolog 2 antibody
    • Essential meiotic endonuclease 1, S. pombe, homolog of, 1 antibody
    • FLJ31364 antibody
    • hMMS4 antibody
    • homolog of yeast EME1 endonuclease antibody
    • MGC106543 antibody
    • MMS4 antibody
    • MMS4 homolog antibody
    • MMS4L antibody
    see all

画像

  • Anti-EME1 antibody (ab87170) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 25 kDa,32 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab87170 has not yet been referenced specifically in any publications.

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