Anti-Embigin homolog 抗体 (ab72195)

製品の概要

  • 製品名Anti-Embigin homolog antibody
    Embigin homolog 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Embigin homolog
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a portion of amino acids 130-180 of Human Embigin homolog

  • ポジティブ・コントロール
    • MCF7 and Raw cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

ターゲット情報

Anti-Embigin homolog antibody 画像

  • All lanes : Anti-Embigin homolog antibody (ab72195) at 0.25 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate with immunising peptide
    Lane 3 : Raw cell lysate


    Predicted band size : 37 kDa
    Observed band size : 37 kDa

Anti-Embigin homolog antibody (ab72195) 使用論文

ab72195 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HUES7 ES cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification HUES7 ES cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Feb 02 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEPG2)
Specification HEPG2
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
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投稿 Nov 18 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 20 µg
Specification HEPG2
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Nov 13 2015

Application Western blot
Sample Human Cell lysate - whole cell (human embryonic stem cells)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 100000 cells
Specification human embryonic stem cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Aug 18 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample Mouse Cell (Mouse embryonic stem cell)
Specification Mouse embryonic stem cell
Permeabilization Yes - 0.1% Triton X
Fixative methanol:acetone 1:1
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投稿 Sep 23 2014

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"