The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/40. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Use at an assay dependent dilution.
Use at an assay dependent dilution. Predicted molecular weight: 34 kDa.
Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
Contains 1 tRNA-binding domain.
Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
Nucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
Small inducible cytokine subfamily E member 1 antibody
Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
Small inducible cytokine subfamily E, member 1, formerly antibody
Western blot - EMAP II antibody [546-2] (ab15693)
Lanes 1, 2, 3, 4, 6, 7, 8 & 9 : Anti-EMAP II antibody [546-2] (ab15693) at 1/1000 dilution Lane 5 : Lane 10 :
Lane 1 : HeLa Cytoplasmic Extract (0.625ug) Lane 2 : HeLa Cytoplasmic Extract (1.25ug) Lane 3 : HeLa Cytoplasmic Extract (2.5ug) Lane 4 : HeLa Cytoplasmic Extract (5ug) Lane 5 : High Range Rainbow MWMs Lane 6 : HeLa Nuclear Extract (0.625ug) Lane 7 : HeLa Nuclear Extract (1.25ug) Lane 8 : HeLa Nuclear Extract (2.5ug) Lane 9 : HeLa Nuclear Extract (5ug) Lane 10 : High Range Rainbow MWMs