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Our Abpromise guarantee covers the use of ab15693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/40. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 34 kDa.|
Samples were run on a 4-12% polyacrylamide gradient gel (SDS, Bis-Tris). Blocking and secondary antibody incubations were at room temperature. Primary antibody incubation was at 4°C.Primary and secondary antibodies were diluted in TBST containing 5% milk.
The predominant band observed was 43 kDa, consistent with the expected mobility of pro-EmapII/p43. The 21kDa "cleaved" form, EmapII, was not observed most likely because the cells were not treated to induce cleavage of pro-EmapII to release EmapII. The identity of the 50kDa "nonspecific" band is not known, but it does not appear as prominent when a different EmapII antibody (ab10605) is used.
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