製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to Elongin A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Fusion protein:

    VKDIRNNKYRIPYRMLYSKYQQEVEKKQEESAERLRLEMQKLQQEREKKQ TIVVDHTVYFKKRNTKKTTRLHNEPHSQLYMKSLKDHESRLKHFKDGGFN

    , corresponding to amino acids 115/214 of S. cerevisiae Elongin A

  • 特記事項
    Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Constituents: 50% Glycerol
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 44 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能
    SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
  • 配列類似性
    Contains 1 F-box domain.
    Contains 1 TFIIS N-terminal domain.
  • ドメイン
    The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Ela1 antibody
      • Elo A antibody
      • EloA antibody
      • ELOA1_HUMAN antibody
      • Elongin 110 kDa subunit antibody
      • Elongin-A antibody
      • ElonginA antibody
      • RNA polymerase II transcription factor SIII subunit A1 antibody
      • SIII antibody
      • SIII p110 antibody
      • TCEB 3 antibody
      • Tceb3 antibody
      • TCEB3A antibody
      • Transcription elongation factor B (SIII) polypeptide 3 (110kD) antibody
      • Transcription elongation factor B (SIII) polypeptide 3 antibody
      • Transcription elongation factor B alpha subunit antibody
      • Transcription elongation factor B polypeptide 3 antibody
      see all

    プロトコール

    参考文献

    ab22093 has not yet been referenced specifically in any publications.

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