製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ELMO1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human ELMO1.

    (Peptide available as ab72858.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Jurkat and MOLT4 Whole Cell Lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

ターゲット情報

  • 機能
    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • 組織特異性
    Widely expressed, with a higher expression in the spleen and placenta.
  • 配列類似性
    Contains 1 ELMO domain.
    Contains 1 PH domain.
  • 翻訳後修飾
    Phosphorylated by HCK.
  • 細胞内局在
    Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CED 12 antibody
    • Ced 12 homolog 1 antibody
    • Ced 12 homolog antibody
    • CED-12 antibody
    • CED12 antibody
    • Ced12 homolog 1 antibody
    • Ced12 homolog antibody
    • ELMO 1 antibody
    • ELMO-1 antibody
    • Elmo1 antibody
    • ELMO1_HUMAN antibody
    • Engulfment and cell motility 1 antibody
    • Engulfment and cell motility protein 1 antibody
    • KIAA0281 antibody
    • MGC126406 antibody
    • Protein ced-12 homolog antibody
    see all

画像

  • All lanes : Anti-ELMO1 antibody (ab72859) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 50 kDa,73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

プロトコール

参考文献

ab72859 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Jurkat Ms Brain Ms Spleen Ms Testis)
Loading amount
20 µg
Specification
Jurkat Ms Brain Ms Spleen Ms Testis
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

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投稿 May 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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