製品の概要

  • 製品名Anti-ELMO1 antibody
    ELMO1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to ELMO1
  • 特異性This antibody is expected to recognise both human isoforms.
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Cow, Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    PKEPSNYDFVYDCN

    , corresponding to C terminal amino acids 714-727 of Human ELMO1.

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution. PubMed: 18662984
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Can be blocked with Human ELMO1 peptide (ab22878). Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa).
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • 組織特異性Widely expressed, with a higher expression in the spleen and placenta.
  • 配列類似性Contains 1 ELMO domain.
    Contains 1 PH domain.
  • 翻訳後修飾Phosphorylated by HCK.
  • 細胞内局在Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CED 12 antibody
    • Ced 12 homolog 1 antibody
    • Ced 12 homolog antibody
    • CED-12 antibody
    • CED12 antibody
    • Ced12 homolog 1 antibody
    • Ced12 homolog antibody
    • ELMO 1 antibody
    • ELMO-1 antibody
    • Elmo1 antibody
    • ELMO1_HUMAN antibody
    • Engulfment and cell motility 1 antibody
    • Engulfment and cell motility protein 1 antibody
    • KIAA0281 antibody
    • MGC126406 antibody
    • Protein ced-12 homolog antibody
    see all

Anti-ELMO1 antibody 画像

  • Ab2239 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  •     Lane 1-3: whole cell lysate (differentiated HL60 cells)

    Lane 4: Protein molculare standard (100, 75 and 50kDa)

    Lane 5: Immunoprecipitation with normal IgG (mock)

    Lane 6 and 7: Immunoprecipitation with ab2239 antibody

    Immunoprecipiated with ab2239 1.5 ug/sample (about 400 ug total protein). Immunoblotted with polyclonal rabbit anti-Dock2  or ab2239 antibody.

    See Abreview

  • ab2239 staining ELMO1 in human HL60 cells by Immunocytochemistry/ Immunofluoresecence.  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized in 0.2% Triton X-100 in PBS for 5 minutes, blocked in 10% normal donkey serum for 30 minutes and incubated with primary antibodies for 2 hours at room temperature. After washing three times with 0.1% Tween 20 in PBS, the coverslips were incubated with fluorescence-conjugated secondary antibodies for 1 hour.

  • IHC image of ab2239 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2239, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ELMO1 antibody (ab2239) 使用論文

This product has been referenced in:
  • Capala ME  et al. ELMO1 is upregulated in AML CD34+ stem/progenitor cells, mediates chemotaxis and predicts poor prognosis in normal karyotype AML. PLoS One 9:e111568 (2014). WB ; Human . Read more (PubMed: 25360637) »
  • Toret CP  et al. An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion. J Cell Biol 207:577-87 (2014). Read more (PubMed: 25452388) »

See all 9 Publications for this product

Product Wall

Thank you for your inquiry.
I do believe the band you're seeing at 50 kDa is specific for ELMO1. The protein has 3 isoforms, according to SwissProt, at 84, 50, and 29 kDa.
http://www.uniprot.org/uniprot/Q92556
So it may be that your samp...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (differentiated HL60)
Total protein in input 1.5e+007 cells
Specification differentiated HL60
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Apr 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (differentiated HL60)
Loading amount 20 µg
Specification differentiated HL60
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Jiqing Sai

Verified customer

投稿 Feb 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"