Anti-ELK4 抗体 (ab86002)
Key features and details
- Rabbit polyclonal to ELK4
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-ELK4 antibody -
製品の詳細
Rabbit polyclonal to ELK4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Three synthetic peptides derived from human ELK4, conjugated to KLH.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Thimerosal (merthiolate) -
Concentration information loading...
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精製度
Whole antiserum -
特記事項(精製)
Crude serum, final bleed -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86002の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 47 kDa.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 47 kDa. |
ターゲット情報
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機能
Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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別名
- Elk4 antibody
- ELK4_HUMAN antibody
- ETS domain containing protein Elk4 antibody
see all
画像
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All lanes : Anti-ELK4 antibody (ab86002) at 1/1000 dilution (in 5% non-fat milk-PBST solution incubated for 60 minutes)
Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution)
Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution)
Secondary
All lanes : anti-Rabbit IgG HRP conjugated incubated for 60 minutes at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
Gel concentration: 10%
The actual band molecular weight is the result of the target protein ELK4 (47 kDa) attached to a tag (14 kDa).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab86002 は 1 報の論文で使用されています。
- Mao X et al. ELK4-mediated lncRNA SNHG22 promotes gastric cancer progression through interacting with EZH2 and regulating miR-200c-3p/Notch1 axis. Cell Death Dis 12:957 (2021). PubMed: 34663788