Anti-ELK1 (phospho T417) 抗体 (ab28817)

製品の概要

  • 製品名Anti-ELK1 (phospho T417) antibody
    ELK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ELK1 (phospho T417)
  • 特異性ab28817 recognises Phospho-ELK-1(Thr417).
  • アプリケーション適用あり: ELISA, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phospho-peptide derived from human ELK-1 around the phosphorylation site of Threonine 417.

  • ポジティブ・コントロール
    • Breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • 組織特異性Lung and testis.
  • 配列類似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻訳後修飾Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 (phospho T417) antibody 画像

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-ELK-1(Thr417) Antibody (ab28817); Right: The same antibody preincubated with synthesized phosphopeptide.

Anti-ELK1 (phospho T417) antibody (ab28817) 使用論文

ab28817 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Human Cell lysate - whole cell (A431 Cells)
Specification A431 Cells
Treatment 30uM etoposide for 30 hrs
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 17 2013

Thank you for your enquiry and your interest in our products.

I regret to inform that you that the blocking peptide (neither the immunizing peptides) are commercially available.

Apologies!

If you need any further assistanc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"