製品の概要

  • 製品名
    Anti-ELK1 (phospho S389) antibody
    ELK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ELK1 (phospho S389)
  • 特異性
    ab28818 recognises phospho-ELK-1(Ser389).
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phospho-peptide derived from human ELK-1 around the phosphorylation site of Serine 389.

  • ポジティブ・コントロール
    • Breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 45 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • 組織特異性
    Lung and testis.
  • 配列類似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻訳後修飾
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

画像

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-ELK-1(Ser389) ab28818; Right: The same antibody preincubated with synthesized phosphopeptide.
  • ab28818 staining ELK1 (phospho S389) in human bladder transitional cell carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/50) for 1 hour at 20°C. An undiluted HRP-conjugated Mouse anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Ahmad I  et al. K-Ras and ß-catenin mutations cooperate with Fgfr3 mutations in mice to promote tumorigenesis in the skin and lung, but not in the bladder. Dis Model Mech 4:548-55 (2011). IHC . Read more (PubMed: 21504907) »

See 1 Publication for this product

レビューと Q&A

Thank you for your enquiry and your interest in our products.

I regret to inform that you that the blocking peptide (neither the immunizing peptides) are commercially available.

Apologies!

If you need any further assistanc...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human bladder transitional cell carcinoma)
Specification
human bladder transitional cell carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Username

Dr. Imran Ahmad

Verified customer

投稿 Nov 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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