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RabMAb

Anti-ELK1 抗体 [E277] - ChIP Grade (ab32106)

製品の概要

  • 製品名
    Anti-ELK1 antibody [E277] - ChIP Grade
    ELK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E277] to ELK1 - ChIP Grade
  • 特異性
    This antibody does not cross react with other ETS family members
  • アプリケーション
    適用あり: WB, IHC-P, ICC, IP, Flow Cyt, ChIPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in C terminus of human ELK1.

  • エピトープ
    ab32106 reacts with an epitope located in the C terminal region of ELK1.
  • ポジティブ・コントロール
    • Human breast carcinoma
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50.
ICC 1/100.
IP 1/80.
Flow Cyt 1/20.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP Use at an assay dependent concentration. PubMed: 22589737

ターゲット情報

  • 機能
    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • 組織特異性
    Lung and testis.
  • 配列類似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻訳後修飾
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 antibody [E277] - ChIP Grade 画像

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab32106 at a dilution of 1/50
  • Anti-ELK1 antibody [E277] - ChIP Grade (ab32106) at 1/500 dilution

    Predicted band size : 45 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ELK1 with unpurified ab32106 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Anti-ELK1 antibody [E277] - ChIP Grade (ab32106) 使用論文

This product has been referenced in:
  • Shuang T  et al. NF-?B1, c-Rel, and ELK1 inhibit miR-134 expression leading to TAB1 upregulation in paclitaxel-resistant human ovarian cancer. Oncotarget 8:24853-24868 (2017). ChIP . Read more (PubMed: 28206956) »
  • Osmanbeyoglu HU  et al. Pancancer modelling predicts the context-specific impact of somatic mutations on transcriptional programs. Nat Commun 8:14249 (2017). Read more (PubMed: 28139702) »

See all 19 Publications for this product

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The concentration of this lot is 0.2180 mg/ml

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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