Anti-Elf4/MEF 抗体 (ab96075)
Key features and details
- Rabbit polyclonal to Elf4/MEF
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Elf4/MEF antibody
Elf4/MEF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Elf4/MEF -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: Mouse testis; Mouse lung; Mouse spleen as well as the following whole cell lysates: NIH3T3; K562.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96075の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa).
|
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa). |
ターゲット情報
-
機能
Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner. Can also promote celular transformation through inhibition of the p16 pathway. -
組織特異性
Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain. -
関連疾患
A chromosomal aberration involving ELF4 is a cause of acute myeloid leukemia (AML). Translocation t(X;21)(q25-26;q22) with ERG. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
細胞内局在
Nucleus > PML body. Accumulation into PML nuclear bodies is mediated by PML. - Information by UniProt
-
参照データベース
- Entrez Gene: 2000 Human
- Entrez Gene: 56501 Mouse
- SwissProt: Q99607 Human
- SwissProt: Q9Z2U4 Mouse
- Unigene: 271940 Human
- Unigene: 36808 Mouse
-
別名
- E74-like factor 4 antibody
- ELF4 antibody
- ELF4_HUMAN antibody
see all
画像
-
All lanes : Anti-Elf4/MEF antibody (ab96075) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 3 : Spleen (Mouse) Tissue Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab96075 は 1 報の論文で使用されています。
- Shi L et al. ELF4 is critical to zygotic gene activation and epigenetic reprogramming during early embryonic development in pigs. Front Vet Sci 9:954601 (2022). PubMed: 35928113