製品の概要

  • 製品名Anti-ELAVL4 antibody
    ELAVL4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ELAVL4
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Rabbit, Chicken, Human, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse ELAVL4.

  • ポジティブ・コントロール
    • This antibody gave a positive signal within Western Blot in the following tissue lysates: Mouse Brain P7; Rat Brain P7; Mouse Brain P0; Rat Brain P0. This antibody gave a positive result in IHC in the following FFPE tissue: Human hippocampus.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Abcam recommends using milk as the blocking agent (3%).

ターゲット情報

  • 機能May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.
  • 組織特異性Brain.
  • 配列類似性Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • 翻訳後修飾Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
    • ELAV L4 antibody
    • ELAV like 4 antibody
    • ELAV like protein 4 antibody
    • ELAV-like protein 4 antibody
    • ELAV4_HUMAN antibody
    • Elavl4 antibody
    • Embryonic lethal abnormal vision Drosophila homolog of like 4 antibody
    • Hu antigen D antibody
    • Hu-antigen D antibody
    • HuD antibody
    • Paraneoplastic encephalomyelitis antigen HuD antibody
    • PNEM antibody
    see all

Anti-ELAVL4 antibody 画像

  • ICC/IF image of ab96474 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96474 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ELAVL4 antibody (ab96474) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : P7 Mouse Brain Tissue Lysate Lab Lysate
    Lane 2 : P7 Rat Brain Rat Tissue Lysate
    Lane 3 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 4 : P0 Rat Brain Rat Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 40,42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • IHC image of ELAVL4 staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ELAVL4 antibody (ab96474) 使用論文

ab96474 has not yet been referenced specifically in any publications.

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