製品の概要

  • 製品名Anti-Elastin antibody
    Elastin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Elastin
  • アプリケーション適用あり: IHC-FoFr, IHC-Fr, ICC/IF, IHC-P, ELISAmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Chicken, Dog, Human, Pig
  • 免疫原

    Elastins pooled from pig, human, dog chicken, rat and cow.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 20138661
IHC-Fr Use at an assay dependent concentration. PubMed: 22884813
ICC/IF Use at an assay dependent concentration. PubMed: 22140132
IHC-P Use at an assay dependent concentration.
ELISA 1/1000 - 1/2000.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
    • 組織特異性Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
    • 関連疾患Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
      Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
      Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • 配列類似性Belongs to the elastin family.
    • 翻訳後修飾Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
      Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
    • 細胞内局在Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Elastin antibody
      • ELN antibody
      • ELN_HUMAN antibody
      • SVAS antibody
      • Tropoelastin antibody
      • WBS antibody
      • WS antibody
      see all

    Anti-Elastin antibody 画像

    • Immunohistochemical analysis of Bouin’s solution fixed (25cm H2O pressure for 90 min) paraffin-embedded wild type mouse lung (12mo, female) tissue labeling Elastin with ab21610 at 1/50 dilution, followed by HRP-conjugated secondary antibody.

      Left Panel: No antibody control.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH-3T3 cells labeling Elastin with ab21610 at 1/200 dilution, followed by a FITC-conjugated secondary antibody. The nuclear counter stain is DAPI (blue).

    • Immunohistochemical analysis of frozen microfibrous vascular grafts from rat carotid artery tissue, staining Elastin with ab21610.

    Anti-Elastin antibody (ab21610) 使用論文

    This product has been referenced in:
    • Lichtenberger BM  et al. Epidermal ß-catenin activation remodels the dermis via paracrine signalling to distinct fibroblast lineages. Nat Commun 7:10537 (2016). IHC . Read more (PubMed: 26837596) »
    • Fang CL  et al. Dermal Lipogenesis Inhibits Adiponectin Production in Human Dermal Fibroblasts while Exogenous Adiponectin Administration Prevents against UVA-Induced Dermal Matrix Degradation in Human Skin. Int J Mol Sci 17:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27428951) »

    See all 15 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Fibrotic Skin)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
    Permeabilization No
    Specification Fibrotic Skin
    Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative 10% NBF
    Username

    Ms. Premier Laboratory

    Verified customer

    投稿 Apr 05 2016

    Thank you for contacting us and your interest in our products.
    The three antibodies you mentioned would all be expected to recognise the extracellular elastin. The mouse monoclonals ab9519 and ab97963 are of the same clone (BA-4) and has been shown...

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    Thank you for your enquiry.

    I can confirm that ab21640 and ab9519 are sold as whole antiseruma and ascites respectively. Unpurified antibodies, such as these sold as whole antiserum, ascites or tissue culture supernatant will not have a conc...

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    Thank you for contacting us.
    Your credit note ID is ****.
    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purcha...

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    Thank you for your message. I am sorry to hear you have previouslyhad difficultywith two of our antibodies.

    I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible for us to keep small sample sizes of...

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    Thank you for contacting Abcam.
    I am sorry about all of the issues you are having with ab21610 in western blotting. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples. Since I do no...

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    Thank you for contacting Abcam regarding ab21610.


    I can confirm that this antibody will also react with tropoelastin.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questi...

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    Thank you for your inquiry.

    The lab said that they would think ab21607 & ab21610 would recognize the precursor of elastin, aka tropoelastin, but this hasn't been specifically tested.

    I think I found an antibody that would work for...

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    Merci de votre appel et de votre intérêt pour nos anticorps contre l'élastine. Comme discuté au téléphone, nous n'avons malheureusement pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester un de nos anticorps...

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    Thank you for your enquiry. This antibody is supplied as whole antiserum, and as such it does not contain storage buffer. It is not possible to determine antibody concentration, since whole serum contains many other host serum proteins besides the ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"