製品の概要

  • 製品名Anti-Elastin antibody [10B8]
    Elastin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10B8] to Elastin
  • アプリケーション適用あり: IP, ELISA, ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Dog, Human
    交差が予測される動物種: Goat, Chicken, Cow
  • 免疫原

    Tropoelastin from 17-day old lathyric chick embryo aortae, extracted and purified according to the method of Rich and Foster (1982) Meth. Enzymol. 82: 665-673.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in IHC on formalin-fixed, paraffin-embedded human skin tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa. PubMed: 25323858

ターゲット情報

  • 機能Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • 組織特異性Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • 関連疾患Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 配列類似性Belongs to the elastin family.
  • 翻訳後修飾Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • 細胞内局在Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    • WBS antibody
    • WS antibody
    see all

Anti-Elastin antibody [10B8] 画像

  • IHC image of ab77804 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77804 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab77804 staining dog lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes, followed by staining with ab77804 at 1/100 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

Anti-Elastin antibody [10B8] (ab77804) 使用論文

This product has been referenced in:
  • Zhao N  et al. MicroRNA miR145 regulates TGFBR2 expression and matrix synthesis in vascular smooth muscle cells. Circ Res 116:23-34 (2015). WB . Read more (PubMed: 25323858) »
  • Wanjare M  et al. Derivation and maturation of synthetic and contractile vascular smooth muscle cells from human pluripotent stem cells. Cardiovasc Res 97:321-30 (2013). ICC/IF ; Human . Read more (PubMed: 23060134) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer (pH6)
Permeabilization No
Specification Lung
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Tissue lysate - whole (Aorta)
Total protein in input 100 µg
Specification Aorta
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Mar 29 2013

Thank you for contacting us.

Unfortunately, we can only give the discount code for a new application e.g. sandwich ELISA, but not for a new variation of an established application.

I hope this information is still helpful to you. Plea...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Goat Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Apr 13 2012

Vielen Dank für Ihren Anruf.


Ich habe einen Proteinsequenz Vergleich zwischen den Immunogenen derMaus Klone [BA-4] und[10B8] (ab9519, ab97963und ab77804)durchgeführt, und folgendes hat sich ergeben: Die Übereinstimmung zw...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 02 2012

Thanks for your call today and for letting us know about the trouble with ab77804.

As we discussed, I'm sending a free of charge vial ofab21607 which should arrive tomorrow on the order numbr ***.

Please keep me updated with the res...

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Thank you for your reply. I can confirm that ab21607 alpha Elastin antibody has been tested and guaranteed for IHC-P and human and mouse samples. Therefore, this would be a suitable alternative antibody.  I would like to reassure you that we mo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"