製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報
    ICC/IF: Use at a concentration of 1-5 µg/ml.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 13 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
    • 配列類似性
      Contains 1 WAP domain.
    • ドメイン
      Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cementoin antibody
      • ELAF_HUMAN antibody
      • Elafin antibody
      • Elastase Specific Inhibitor antibody
      • Elastase-specific inhibitor antibody
      • ES 1 antibody
      • ESI antibody
      • Peptidase inhibitor 3 antibody
      • Peptidase inhibitor 3, skin derived antibody
      • PI 3 antibody
      • PI-3 antibody
      • PI3 antibody
      • Pre elafin antibody
      • Protease inhibitor 3 skin derived antibody
      • Protease inhibitor 3, skin derived (SKALP) antibody
      • Protease inhibitor WAP3 antibody
      • SKALP antibody
      • Skin derived Anti leukoproteinase antibody
      • Skin-derived antileukoproteinase antibody
      • Trappin 2 antibody
      • WAP four disulfide core domain 14 antibody
      • WAP four disulfide core domain protein 14 antibody
      • WAP four-disulfide core domain protein 14 antibody
      • WAP3 antibody
      • WFDC14 antibody
      see all

    画像

    • All lanes : Anti-Elafin/Skalp antibody (ab46774) at 1/1000 dilution

      Lane 1 : recombinant Human Elafin at 0.05 µg
      Lane 2 : recombinant Human Elafin at 0.01 µg
      Lane 3 : recombinant Human Elafin at 0.001 µg


      Predicted band size : 13 kDa
      Observed band size : 14 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 18 kDa (possible dimer).
    • ICC/IF image of ab46774 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46774, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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    参考文献

    ab46774 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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