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Our Abpromise guarantee covers the use of ab2483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent dilution. PubMed: 19074263|
|WB||Use at an assay dependent dilution. Preliminary experiments gave an approx 52kDa band in K562 cell lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 38kDa according to NP_006554. The 52kDa band was successfully blocked by incubation with the immunizing peptide|