Anti-eIF5A 抗体 [EP527Y] (ab32407)


  • 製品名Anti-eIF5A antibody [EP527Y]
    eIF5A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP527Y] to eIF5A
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF5A aa 50-150 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: Jurkat cell lysates. IHC-P: human urinary bladder carcinoma.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab32407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 機能mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
    • 組織特異性Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
    • 配列類似性Belongs to the eIF-5A family.
    • 翻訳後修飾eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
    • 細胞内局在Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
    • Information by UniProt
    • 参照データベース
    • 別名
      • eIF 4D antibody
      • eIF 5A 1 antibody
      • EIF 5A antibody
      • eIF 5A1 antibody
      • eIF-4D antibody
      • eIF-5A antibody
      • eIF-5A-1 antibody
      • eIF-5A1 antibody
      • eIF4D antibody
      • eif5a antibody
      • EIF5A1 antibody
      • eIF5AI antibody
      • Eukaryotic initiation factor 5A antibody
      • Eukaryotic initiation factor 5A isoform 1 antibody
      • Eukaryotic translation initiation factor 5A 1 antibody
      • Eukaryotic translation initiation factor 5A antibody
      • Eukaryotic translation initiation factor 5A-1 antibody
      • IF5A1_HUMAN antibody
      • MGC104255 antibody
      • MGC99547 antibody
      • Rev binding factor antibody
      • Rev-binding factor antibody
      • uORF A antibody
      • uORF antibody
      see all

    Anti-eIF5A antibody [EP527Y] 画像

    • Anti-eIF5A antibody [EP527Y] (ab32407) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • Ab32407, at a dilution of 1/250, staining eIF5A in paraffin embedded human urinary bladder carcinoma tissue sections by Immunohistochemistry.
    • Overlay histogram showing Jurkat cells stained with ab32407 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32407, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-eIF5A antibody [EP527Y] (ab32407) 使用論文

    ab32407 has not yet been referenced specifically in any publications.

    Product Wall

    We have tested ab32407 in IP and in our hands we could not get this to work hence why we have stated on the datasheet that this antibody is unsuitable for use in this application.

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