製品の概要

  • 製品名Anti-EIF5 antibody
    EIF5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to EIF5
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, Human EIF5 (432 aa) with 26 kDa enzyme tag (AAH32866).

  • ポジティブ・コントロール
    • Recombinant full length human EIF5.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged EIF5 is approximately 10ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 49 kDa).

ターゲット情報

  • 機能Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
  • 配列類似性Belongs to the eIF-2-beta/eIF-5 family.
    Contains 1 W2 domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2810011H21Rik antibody
    • D12Ertd549e antibody
    • EIF 5 antibody
    • EIF 5A antibody
    • eIF-5 antibody
    • Eif5 antibody
    • Eukaryotic initiation factor 5 antibody
    • Eukaryotic translation initiation factor 5 antibody
    • IF5_HUMAN antibody
    • MGC36374 antibody
    • MGC36509 antibody
    see all

Anti-EIF5 antibody 画像

  • Anti-EIF5 antibody (ab76633) at 5 µg/ml + Recombinant full length human EIF5 with 26 kDa tag, at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 49 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)

Anti-EIF5 antibody (ab76633) 使用論文

ab76633 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab76633.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"