製品の概要

  • 製品名Anti-eIF4H antibody
    eIF4H 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4H
  • アプリケーション適用あり: IHC-P, WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    A region between residue 198 and 248 of human eIF4H using the numbering given in entry NP_071496.1

  • ポジティブ・コントロール
    • HeLa cells lysate 293T cells lysate
  • 特記事項Shelf Life: 1 year from date of receipt

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Affinity purified using an epitope specific to eIF4H immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab86179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
    • 組織特異性The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
    • 関連疾患Note=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
    • 細胞内局在Cytoplasm > perinuclear region.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AU018978 antibody
      • D5Ertd355e antibody
      • E430026L18Rik antibody
      • Ef4h antibody
      • eIF 4H antibody
      • eIF-4H antibody
      • EIF4H antibody
      • Eukaryotic translation initiation factor 4H antibody
      • IF4H_HUMAN antibody
      • KIAA0038 antibody
      • WBSCR1 antibody
      • Williams Beuren syndrome chromosomal region 1 protein homolog antibody
      • Williams Beuren syndrome chromosome region 1 antibody
      • Williams-Beuren syndrome chromosomal region 1 protein antibody
      • Williams-Beuren syndrome chromosome region 1 antibody
      • WSCR1 antibody
      see all

    Anti-eIF4H antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling eIF4H with ab86179 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-eIF4H antibody (ab86179) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 27 kDa


      Exposure time : 3 minutes

    Anti-eIF4H antibody (ab86179) 使用論文

    ab86179 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"