Anti-eIF4H 抗体 (ab86179)
Key features and details
- Rabbit polyclonal to eIF4H
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-eIF4H antibody -
製品の詳細
Rabbit polyclonal to eIF4H -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human eIF4H aa 150-250.
Database link: Q15056 -
ポジティブ・コントロール
- HeLa cells lysate 293T cells lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. The product can be stored for up to 12 months. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity purified using an epitope specific to eIF4H immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86179の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
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特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 27 kDa. |
ターゲット情報
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機能
Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA. -
組織特異性
The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney. -
関連疾患
Note=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
配列類似性
Contains 1 RRM (RNA recognition motif) domain. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 7458 Human
- Entrez Gene: 22384 Mouse
- Entrez Gene: 288599 Rat
- Omim: 603431 Human
- SwissProt: Q15056 Human
- SwissProt: Q9WUK2 Mouse
- SwissProt: Q5RBR8 Orangutan
- SwissProt: Q5XI72 Rat
see all -
別名
- AU018978 antibody
- D5Ertd355e antibody
- E430026L18Rik antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling eIF4H with ab86179 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-eIF4H antibody (ab86179) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab86179 は論文での使用が確認できていません。