製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to eIF4GII
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 1152-1201 (

    MRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRES

    ) of human EIF4G3 (NP_003751).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab83330 is purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titer in peptide based assay is 1/1562500.

ターゲット情報

  • 機能
    Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.
  • 配列類似性
    Belongs to the eukaryotic initiation factor 4G family.
    Contains 5 HEAT repeats.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • 翻訳後修飾
    Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
  • Information by UniProt
  • 参照データベース
  • 別名
    • eIF-4-gamma 3 antibody
    • eIF-4-gamma II antibody
    • eIF-4G 3 antibody
    • eIF4G 3 antibody
    • EIF4G3 antibody
    • eIF4GII antibody
    • Eukaryotic translation initiation factor 4 gamma 3 antibody
    • eukaryotic translation initiation factor 4 gamma, 3 antibody
    • eukaryotic translation initiation factor-4 gamma, 3 antibody
    • IF4G3_HUMAN antibody
    see all

Anti-eIF4GII antibody 画像

  • Anti-eIF4GII antibody (ab83330) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 177 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

プロトコール

Anti-eIF4GII antibody (ab83330) 使用論文

ab83330 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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