製品の概要

  • 製品名Anti-eIF4ENIF1 antibody
    eIF4ENIF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4ENIF1
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 925 and the C-terminus (residue 985) of Human eIF4ENIF1 (NP_062817.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate, 293T whole cell lysate and mouse NIH3T3 whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 108 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
  • 組織特異性Widely expressed.
  • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2610509L04Rik antibody
    • 4E T antibody
    • 4E-T antibody
    • 4ET antibody
    • 4ET_HUMAN antibody
    • A930019J01Rik antibody
    • AA410001 antibody
    • AU021239 antibody
    • Clast 4 antibody
    • Clast4 antibody
    • D11Ertd166e antibody
    • eIF4E transporter antibody
    • Eif4enif1 antibody
    • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
    • Eukaryotic translation initiation factor 4E transporter antibody
    • FLJ21601 antibody
    • FLJ26551 antibody
    • OTTMUSP00000000698 antibody
    • OTTMUSP00000005193 antibody
    • OTTMUSP00000005194 antibody
    • OTTMUSP00000036075 antibody
    see all

Anti-eIF4ENIF1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling eIF4ENIF1 with ab95030 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-eIF4ENIF1 antibody (ab95030) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 108 kDa


    Exposure time : 3 minutes
  • Detection of eIF4ENIF1 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab95030 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-eIF4ENIF1 antibody (ab95030) 使用論文

ab95030 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95030.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"