製品の概要

  • 製品名Anti-eIF4ENIF1 antibody
    eIF4ENIF1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to eIF4ENIF1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Baboon
  • 免疫原

    Recombinant fragment: NPRPGTPLHL AMVQQQLQRS VLHPPGSGSH AAAVSVQTTP QNVPSRSGLP HMHSQLEHRP SQRSSSPVGL AKWFGSDVLQ QPLPSMPAKV ISVDELEYRQ, corresponding to amino acids 886 - 985 of human eIF4ENIF1 (NP_062817) with tag.

  • ポジティブ・コントロール
    • Partial recombinant human eIF4ENIF1 protein with tag ; eIF4ENIF1 transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 108 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
  • 組織特異性Widely expressed.
  • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2610509L04Rik antibody
    • 4E T antibody
    • 4E-T antibody
    • 4ET antibody
    • 4ET_HUMAN antibody
    • A930019J01Rik antibody
    • AA410001 antibody
    • AU021239 antibody
    • Clast 4 antibody
    • Clast4 antibody
    • D11Ertd166e antibody
    • eIF4E transporter antibody
    • Eif4enif1 antibody
    • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
    • Eukaryotic translation initiation factor 4E transporter antibody
    • FLJ21601 antibody
    • FLJ26551 antibody
    • OTTMUSP00000000698 antibody
    • OTTMUSP00000005193 antibody
    • OTTMUSP00000005194 antibody
    • OTTMUSP00000036075 antibody
    see all

Anti-eIF4ENIF1 antibody 画像

  • Anti-eIF4ENIF1 antibody (ab77027) at 1 µg/ml + recombinant human eIF4ENIF1 protein fragment with a 26 kDa tag at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 108 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-eIF4ENIF1 antibody (ab77027) at 1 µg/ml

    Lane 1 : eIF4ENIF1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 108 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

Anti-eIF4ENIF1 antibody (ab77027) 使用論文

ab77027 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293T, HeLa)
Loading amount 20 µg
Specification 293T, HeLa
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"