The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use 5-10 µl, using HeLa cell lysate.
ICC/IF: Use at a concentration of 5 - 10 µg/ml, using paraformaldehyde-fixed HEK-293T cells.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa using HeLa cell lysate. Staining of eIF4ENIF1 in immunoblotting is specifically inhibited by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
Kamenska A et al. The DDX6-4E-T interaction mediates translational repression and P-body assembly. Nucleic Acids Res44:6318-34 (2016).
Read more (PubMed: 27342281) »
Cargnello M et al. Phosphorylation of the eukaryotic translation initiation factor 4E-transporter (4E-T) by c-Jun N-terminal kinase promotes stress-dependent P-body assembly. Mol Cell Biol32:4572-84 (2012).
Read more (PubMed: 22966201) »