製品の概要

  • 製品名Anti-eIF4ENIF1 antibody
    eIF4ENIF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4ENIF1
  • 特異性ab55880 specifically recognizes human eIF4ENIF1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide:

    KPQVDRTRDGFRNRQ

    conjugated to KLH, corresponding to amino acids 650-664 of Human eIF4ENIF1

  • ポジティブ・コントロール
    • HeLa cytoplasmic or nuclear lysate
  • 特記事項If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab55880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use 5µl for 1010 cells.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 108 kDa).
    Staining of eIF4ENIF1 in immunoblotting is specifically inhibited by the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
    • 組織特異性Widely expressed.
    • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
    • Information by UniProt
    • 参照データベース
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    Anti-eIF4ENIF1 antibody 画像

    • All lanes : Anti-eIF4ENIF1 antibody (ab55880) at 2 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : K562 cell lysate

      Secondary
      HRP conjugated Goat-anti Rabbit IgG
      Developed using the ECL technique

      Predicted band size : 108 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    Anti-eIF4ENIF1 antibody (ab55880) 使用論文

    ab55880 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab55880.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"