The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use 5µl for 1010 cells.
WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 108 kDa).
Staining of eIF4ENIF1 in immunoblotting is specifically inhibited by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.