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Synthetic peptide: MNNKFDALKD DDSGDHDQNE ENSTQKDGEK EKTERDKNQS SSKRKAVVPG, corresponding to amino acids 1-50 of Human EIF4EL3
Our Abpromise guarantee covers the use of ab50934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:62500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling EIF4EL3 with ab50934 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
ab50934 has not yet been referenced specifically in any publications.