Anti-eIF4EBP1 (phospho T70) 抗体 (ab53687)

製品の概要

  • 製品名Anti-eIF4EBP1 (phospho T70) antibody
    eIF4EBP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4EBP1 (phospho T70)
  • 特異性ab53687 detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 69.
  • アプリケーション適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site threonine 70 (A-K-TP-P-P).

  • ポジティブ・コントロール
    • Human colon carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

ターゲット情報

  • 機能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 配列類似性Belongs to the eIF4E-binding protein family.
  • 翻訳後修飾Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho T70) antibody 画像

  • ab53687 at 1/50 dilution staining eIF4EBP1 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Anti-eIF4EBP1 (phospho T70) antibody (ab53687) 使用論文

ab53687 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Sample Mouse Tissue sections (Brain tissue)
Specification Brain tissue
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Mar 27 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Sample Mouse Cell (Brain tissue)
Specification Brain tissue
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Mar 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"