Anti-eIF4EBP1 (phospho T46) 抗体 (ab27792)

製品の概要

  • 製品名Anti-eIF4EBP1 (phospho T46) antibody
    eIF4EBP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4EBP1 (phospho T46)
  • 特異性Ab27792 recognises eIF4EBP1 that is phosphorylated at threonine 46.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46. The sequence is conserved in human, mouse and rat.

  • ポジティブ・コントロール
    • Human embryonic kidney (HEK293) cells stimulated with EGF or 15% serum, or NIH3T3 cells stimulated with PDGF.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 13 kDa.

ターゲット情報

  • 機能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 配列類似性Belongs to the eIF4E-binding protein family.
  • 翻訳後修飾Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho T46) antibody 画像

  • All lanes : Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two hours at room temperature.)

    Lane 1 : PDGF treated NIH3T3 cell lysate
    Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
    Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate

    Secondary
    goat F(ab)2 anti rabbit IgG HRP conjugate

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

Anti-eIF4EBP1 (phospho T46) antibody (ab27792) 使用論文

ab27792 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab27792.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"