Anti-eIF4EBP1 (phospho S64) 抗体 (ab53686)

製品の概要

  • 製品名
    Anti-eIF4EBP1 (phospho S64) antibody
    eIF4EBP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4EBP1 (phospho S64)
  • 特異性
    ab53686 detects endogenous levels of eIF4EBP1 only when phosphorylated at serine 64.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of serine 64 (R-N-SP-P-V).

  • ポジティブ・コントロール
    • Human colon carcinoma tissue and Jurkat cell extracts treated with Insulin (0.01U/ml, 15mins).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
ELISA 1/1000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 配列類似性
    Belongs to the eIF4E-binding protein family.
  • 翻訳後修飾
    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho S64) antibody 画像

  • All lanes : Anti-eIF4EBP1 (phospho S64) antibody (ab53686) at 1/500 dilution

    Lane 1 : Jurkat cell extract treated with Insulin (0.01U/ml, 15mins)
    Lane 2 : Jurkat cell extract treated with Insulin (0.01U/ml, 15mins) with immunising peptide


    Predicted band size : 13 kDa
    Observed band size : 13 kDa
  • ab53686 at 1/50 dilution staining eIF4EBP1 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Anti-eIF4EBP1 (phospho S64) antibody (ab53686) 使用論文

ab53686 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Sample
Mouse Tissue sections (Brain tissue)
Specification
Brain tissue
Permeabilization
No
Fixative
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投稿 Mar 14 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Sample
Mouse Cell (Brain tissue)
Specification
Brain tissue
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

投稿 Mar 13 2014

Thank you for contacting us. Unfortunately we are unable to determine the percentage sequence homology between human and Litopenaeus vannamei eIF4BP1 sequence. So we will be unable to provide the discount code. We can however reconsider your reques...

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Thank you for contacting us. Could you specify the species you are interested in? Looking forward to hearing from you soon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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