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製品の概要

  • 製品名Anti-eIF4EBP1 antibody [Y330]
    eIF4EBP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y330] to eIF4EBP1
  • 特異性ab32130 recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS.
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, IPmore details
    適用なし: ICC
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-term of human4E-BP1

  • ポジティブ･コントロール
    • K562 cell lysate and human prostate carcinoma
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 15-20 kDa (predicted molecular weight: 12 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
  • 追加情報Is unsuitable for ICC.

    • ターゲット情報

    • 機能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
    • 配列類似性Belongs to the eIF4E-binding protein family.
    • 翻訳後修飾Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • 参照データベース
      • 別名
        • 4E-BP1 antibody
        • 4EBP1 antibody
        • 4EBP1_HUMAN antibody
        • BP 1 antibody
        • eIF4E binding protein 1 antibody
        • eIF4E-binding protein 1 antibody
        • Eif4ebp1 antibody
        • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
        • PHAS-I antibody
        • PHASI antibody
        • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
        see all

      Anti-eIF4EBP1 antibody [Y330] 画像

      • Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)
        Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)


        Predicted band size : 12 kDa

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: eIF4EBP1 knockout HAP1 cell lysate (20 µg)
        Lane 3: Hek293 cell lysate (20 µg)
        Lane 4: K562 cell lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab32130 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab32130 was shown to specifically react with eIF4EBP1 when eIF4EBP1 knockout samples were used. Wild-type and eIF4EBP1 knockout samples were subjected to SDS-PAGE. ab32130 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

      • Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)
        Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)
        Anti-eIF4EBP1 antibody [Y330] (ab32130) at 1/1000 dilution + K562 cell lysate

        Predicted band size : 12 kDa
        Observed band size : 18 kDa (why is the actual band size different from the predicted?)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody [Y330] (ab32130)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody [Y330] (ab32130)
        ab32130, staining human prostate carcinoma by immunohistochemistry, Paraffin embedded tissue

      Anti-eIF4EBP1 antibody [Y330] (ab32130) 使用論文

      This product has been referenced in:
      • Lu Q  et al. Akt inhibition attenuates rasfonin-induced autophagy and apoptosis through the glycolytic pathway in renal cancer cells. Cell Death Dis 6:e2005 (2015). Human . Read more (PubMed: 26633711) »
      • Hu Y  et al. MiR-99a antitumor activity in human breast cancer cells through targeting of mTOR expression. PLoS One 9:e92099 (2014). WB ; Human . Read more (PubMed: 24637915) »

      See all 3 Publications for this product

      ab32130 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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