Anti-eIF4EBP1 抗体 [Y330] (ab32130)
製品の概要
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製品名Anti-eIF4EBP1 antibody [Y330]
eIF4EBP1 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [Y330] to eIF4EBP1
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由来種Rabbit
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特異性ab32130 recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS.
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アプリケーション適用あり: WB, IHC-P, Flow Cyt, IPmore details
適用なし: ICC -
種交差性交差種: Human
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免疫原
A synthetic peptide corresponding to residues near the C-term of human4E-BP1
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ポジティブ･コントロール
- K562 cell lysate and human prostate carcinoma
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... -
精製度IgG fraction
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ポリ/モノモノクローナル
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クローン名Y330
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab32130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 15-20 kDa (predicted molecular weight: 12 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/50. |
ターゲット情報
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機能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
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配列類似性Belongs to the eIF4E-binding protein family.
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翻訳後修飾Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
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参照データベース
- Entrez Gene: 1978 Human
- Omim: 602223 Human
- SwissProt: Q13541 Human
- Unigene: 411641 Human
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別名
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
see all
画像
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![Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)](http://a.abcam.com/ps/products/32/ab32130/Images/ab32130-256326-ab32130KOWB.jpg)
Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: eIF4EBP1 knockout HAP1 cell lysate (20 µg)
Lane 3: Hek293 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32130 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32130 was shown to specifically react with eIF4EBP1 when eIF4EBP1 knockout samples were used. Wild-type and eIF4EBP1 knockout samples were subjected to SDS-PAGE. ab32130 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)](http://a.abcam.com/ps/products/32/ab32130/Images/ab32130-1.JPG)
Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)Anti-eIF4EBP1 antibody [Y330] (ab32130) at 1/1000 dilution + K562 cell lysate
Predicted band size: 12 kDa
Observed band size: 18 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody [Y330] (ab32130)](http://a.abcam.com/ps/products/32/ab32130/Images/ab32130-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody [Y330] (ab32130)ab32130, staining human prostate carcinoma by immunohistochemistry, Paraffin embedded tissue
参考文献
This product has been referenced in:
- Lu Q et al. Akt inhibition attenuates rasfonin-induced autophagy and apoptosis through the glycolytic pathway in renal cancer cells. Cell Death Dis 6:e2005 (2015). Human . Read more (PubMed: 26633711) »
- Hu Y et al. MiR-99a antitumor activity in human breast cancer cells through targeting of mTOR expression. PLoS One 9:e92099 (2014). WB ; Human . Read more (PubMed: 24637915) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"