製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to eIF4EBP1
  • 特異性
    This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
  • アプリケーション
    適用あり: EMSA, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.

  • ポジティブ・コントロール
    • RINm5F insulinoma cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
EMSA Use at an assay dependent concentration. PubMed: 17074885
IHC-P 1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/6000. Detects a band of approximately 20-25 kDa (predicted molecular weight: 13 kDa).
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 配列類似性
    Belongs to the eIF4E-binding protein family.
  • 翻訳後修飾
    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection: DAB.


  • Predicted band size : 13 kDa
    Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of  eIF-4EBP1 in 100µg of RINm5F insulinoma cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine. Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F insulinoma cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.
  • Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 20,22,25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 22,25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • eIF4EBP1 was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to eIF4EBP1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2606.
    Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
    Band: 20kDa; eIF4EBP1

参考文献

This product has been referenced in:
  • Freis P  et al. mTOR inhibitors activate PERK signaling and favor viability of gastrointestinal neuroendocrine cell lines. Oncotarget 8:20974-20987 (2017). WB ; Mouse . Read more (PubMed: 28423496) »
  • Li H  et al. MEN1/Menin regulates milk protein synthesis through mTOR signaling in mammary epithelial cells. Sci Rep 7:5479 (2017). Read more (PubMed: 28710500) »

See all 11 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
10 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 18 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 cell line)
Loading amount
10 µg
Specification
HT1080 cell line
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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