製品の概要

  • 製品名Anti-eIF4E antibody - N-terminal
    eIF4E 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to eIF4E - N-terminal
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the N terminal region of human eIF4E conjugated to KLH

  • ポジティブ・コントロール
    • HL60 cell line lysate and human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • 配列類似性Belongs to the eukaryotic initiation factor 4E family.
  • 翻訳後修飾Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Anti-eIF4E antibody - N-terminal 画像

  • Anti-eIF4E antibody - N-terminal (ab70873) at 1/250 dilution + HL60 cell line lysates at 12.5 µg

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • ab70873 at 1/50 dilution, staining eIF4E in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-eIF4E antibody - N-terminal (ab70873) 使用論文

ab70873 has not yet been referenced specifically in any publications.

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