Anti-eIF4E 抗体 (ab55879)

製品の概要

• 製品名
  Anti-eIF4E antibody
  eIF4E 一次抗体 製品一覧
• 製品の詳細
  Rabbit polyclonal to eIF4E
• アプリケーション
  適用あり: WB, IP, ICCmore details
• 種交差性
  交差種: Mouse, Rat, Human
  
• 免疫原

  Synthetic peptide:

  KTESNQEVAN PEHYIKH

  conjugated to KLH via a C-terminal added cysteine residue, corresponding to amino acids 21-37 of Human eIF4E

  Run BLAST with Run BLAST with
• ポジティブ･コントロール
  • HeLa cell lysates, paraformaldehyde-fixed 3T3 cells.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  Mix suspension via gentle inversion before use.
• バッファー
  Preservative: 15mM Sodium Azide
  Constituents: 0.01M PBS, pH 7.4
• Concentration information loading...
• 精製度
  Immunogen affinity purified
• ポリ/モノ
  ポリクローナル
• アイソタイプ
  IgG
• 研究分野
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • Translation
  • Regulation

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • RNAi

  • Epigenetics and Nuclear Signaling
  • RNAi
  • Eukaryotic Initiation factors (eIF's)

関連製品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

アプリケーション

ターゲット情報

• 機能
  Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
• 配列類似性
  Belongs to the eukaryotic initiation factor 4E family.
• 翻訳後修飾
  Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

• Target information above from: UniProt accession P06730 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 1977 Human
  • Entrez Gene: 13684 Mouse
  • Entrez Gene: 117045 Rat
  • Omim: 133440 Human
  • SwissProt: P06730 Human
  • SwissProt: P63073 Mouse
  • SwissProt: P63074 Rat
  • Unigene: 249718 Human

  • Unigene: 3941 Mouse
  • Unigene: 11275 Rat

  see all
• 別名
  • AUTS19 antibody
  • CBP antibody
  • eIF 4E antibody

  • eIF 4F 25 kDa subunit antibody
  • EIF 4F antibody
  • eIF-4E antibody
  • eIF-4F 25 kDa subunit antibody
  • eIF4E antibody
  • EIF4E1 antibody
  • EIF4EL1 antibody
  • EIF4F antibody
  • Eukaryotic translation initiation factor 4 E antibody
  • Eukaryotic translation initiation factor 4E antibody
  • Eukaryotic translation initiation factor 4E like 1 antibody
  • IF4E_HUMAN antibody
  • Messanger RNA Cap Binding Protein eIF 4E antibody
  • MGC111573 antibody
  • mRNA cap binding protein antibody
  • mRNA cap-binding protein antibody

  see all

プロトコール

• Western blot protocols
• Immunoprecipitation protocols
• Immunocytochemistry & immunofluorescence protocols