製品の概要

  • 製品名
    Anti-eIF4B (phospho S504) antibody [EP2261Y]
    eIF4B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2261Y] to eIF4B (phospho S504)
  • 特異性
    ab75823 detects eIF4B phosphorylated at serine 504.
  • アプリケーション
    適用あり: WB, ICCmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B

  • ポジティブ・コントロール
    • 293T cell lysates, untreated or treated with AP.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP2261Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ICC 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    画像

    • All lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution

      Lane 1 : 293T cell lysate, untreated
      Lane 2 : 293T cell lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    参考文献

    This product has been referenced in:
    • Bettegazzi B  et al. eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology. Sci Rep 7:10563 (2017). Read more (PubMed: 28874824) »

    See 1 Publication for this product

    レビューと Q&A

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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